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Image Search Results
Journal: STAR Protocols
Article Title: Quantifying molecular aggregation by super resolution microscopy within an excitatory synapse from mouse hippocampal neurons
doi: 10.1016/j.xpro.2021.100470
Figure Lengend Snippet: Object-based segmentation of synaptic puncta using IMA in MetaMorph software (A) A source image as confocal PSD95 image selected for specific objects based on length, breadth, and area filters. (B) A display of filtering parameters in IMA. (C) Object data for the synaptic puncta obtained through IMA. (D) A binary IMA object mask created as an output image. (E and F) Dynamic data exchange command for exporting the object data on postsynaptic puncta to an excel file.
Article Snippet: Object-based segmentation of synaptic puncta using
Techniques: Software
Journal: STAR Protocols
Article Title: Quantifying molecular aggregation by super resolution microscopy within an excitatory synapse from mouse hippocampal neurons
doi: 10.1016/j.xpro.2021.100470
Figure Lengend Snippet: Creating ROIs on the filtered objects in MetaMorph software (A) Region menu displaying several options like creating regions around objects or transferring regions from one image to another. (B) Creating ROIs around the objects filtered with IMA object mask as the source image. The ROIs are marked in yellow color.
Article Snippet: Object-based segmentation of synaptic puncta using
Techniques: Software, Transferring
Journal: STAR Protocols
Article Title: Quantifying molecular aggregation by super resolution microscopy within an excitatory synapse from mouse hippocampal neurons
doi: 10.1016/j.xpro.2021.100470
Figure Lengend Snippet: Semiautomated detection of functional zones (PSD) of an excitatory synapse using MetaMorph software (A and C) STED PSD95 image and a corresponding binary IMA object mask with regions created around objects segmented as PSD regions using IMA. The filtered PSDs are marked in green while the region boundaries are shown in yellow. (B and D) The IMA and thresholding parameters employed for filtering PSDs from STED PSD95 image as a source image.
Article Snippet: Object-based segmentation of synaptic puncta using
Techniques: Functional Assay, Software